Getting research of the excretion balance in mice, radioactivity is actually determined from inside the pee, feces, cage washings (aqueous and you will organic), and you will carcasses. Dehydrated feces homogenates, crate dirt, and you may carcass aliquots have been combusted inside the oxygen playing with a Packard Shot Oxidizer. The new carbon-fourteen combusted products was indeed absorbed for the Carbo-SorbR and you will blended with PermafluorR Elizabeth+ h2o scintillant in advance of LSC. Radioactivity is actually mentioned by the LSC having fun with Tri-Carb 2300 (Packard, Dreieich) liquids scintillation surfaces towards the ability to compute satisfy-corrected disintegrations each minute.
In the pet, the entire radioactivity content out-of plasma each pee and you will feces pond was mentioned because of the LSC playing with Tri-Carbohydrate 2100 (PerkinElmer, Rodgau).
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